The neutral comet assay detects double strand DNA damage in selected and unselected human spermatozoa of normospermic donors
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چکیده
منابع مشابه
Diagnosis of endosulfan induced DNA damage in rohu (Labeo rohita, Hamilton) using comet assay
Use of different pesticides in the agriculture sector, in order to boost crop yield within a short time period and low labor, has been tremendously increased since the last decade. Pesticide use has elevated crop yield but has produced a number of pronounced problems regarding environmental and health safety. The continuously deteriorating toxicological effects of these pesticides are not only ...
متن کاملStudies on electron beam induced DNA damage and repair kinetics in lymphocytes by alkaline comet assay
Background: Exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and DNA through radiolysis of cellular water. Since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the DNA damage and repair kinetics would help to predict the radiation sensitivity of cel...
متن کاملRadioadaptive response in peripheral blood leukocytes of occupationally exposed medical staff with investigation of DNA damage by the use of neutral comet assay
Background: ˝Radioadaptive Response˝ is well-documented phenomenon appeared in low dose ionizing radiation received in vitro and in vivo. Occupational exposure has always been a great concern for radiation workers therefore this study was performed to study radioadaptive response in terms of residual DNA double strand breaks as an endpoint in peripheral blood leukocytes of occupationally expose...
متن کاملDiagnosis of endosulfan induced DNA damage in rohu (Labeo rohita, Hamilton) using comet assay
Use of different pesticides in the agriculture sector, in order to boost crop yield within a short time period and low labor, has been tremendously increased since the last decade. Pesticide use has elevated crop yield but has produced a number of pronounced problems regarding environmental and health safety. The continuously deteriorating toxicological effects of these pesticides are not only ...
متن کاملA two-tailed Comet assay for assessing DNA damage in spermatozoa.
DNA fragmentation is considered an important parameter of semen quality, and of significant value as a predictor of male fertility. Poor quality chromatin is closely associated with, and highly indicative of, some fertility problems. Many methodologies to assess DNA fragmentation in spermatozoa are available, but they are all unable to differentiate between single-stranded DNA breaks (SSB) and ...
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ژورنال
عنوان ژورنال: International Journal of Andrology
سال: 2004
ISSN: 0105-6263,1365-2605
DOI: 10.1111/j.1365-2605.2004.00463.x